Whole genome sequencing-based classification of human-related Haemophilus species and detection of antimicrobial resistance genes.

ABSTRACT:

Background

Bacteria belonging to the genus Haemophilus cause a wide range of diseases in humans. Recently, H. influenzae was classified by the WHO as priority pathogen due to the wide spread of ampicillin resistant strains. However, other Haemophilus spp. are often misclassified as H. influenzae. Therefore, we established an accurate and rapid whole genome sequencing (WGS) based classification and serotyping algorithm and combined it with the detection of resistance genes.

Methods

A gene presence/absence-based classification algorithm was developed, which employs the open-source gene-detection tool SRST2 and a new classification database comprising 36 genes, including capsule loci for serotyping. These genes were identified using a comparative genome analysis of 215 strains

SUBMITTER: Diricks M 

PROVIDER: S-EPMC8830169 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature