RNA recovery from specimens of duct-washing cytology performed contemporaneously with mammary ductoscopy.

ABSTRACT:

Objective

Conventional cytological diagnosis including duct-washing cytology (DWC) is sometimes performed using ductal epithelial cells collected during mammary ductoscopy; it is useful for detection of early-stage breast cancer such as ductal carcinoma in situ (DCIS). However, conventional cytological diagnosis focuses exclusively on cellular morphology; false negatives and false positives may be caused by inadequate specimen preparation (triggering cell degeneration) or poor examiner diagnostic skills. Molecular diagnosis using RNA biomarkers is expected to compensate for the weaknesses of cytological diagnosis. We previously employed microarray analysis to identify highly expressed genes in DCIS, suggesting that they may be useful for DCIS diagnosis. Here, we explored whether DW

SUBMITTER: Jikuzono T 

PROVIDER: S-EPMC8830173 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature