SOMSpec as a General Purpose Validated Self-Organising Map Tool for Rapid Protein Secondary Structure Prediction From Infrared Absorbance Data.

ABSTRACT: A protein's structure is the key to its function. As protein structure can vary with environment, it is important to be able to determine it over a wide range of concentrations, temperatures, formulation vehicles, and states. Robust reproducible validated methods are required for applications including batch-batch comparisons of biopharmaceutical products. Circular dichroism is widely used for this purpose, but an alternative is required for concentrations above 10 mg/mL or for solutions with chiral buffer components that absorb far UV light. Infrared (IR) protein absorbance spectra of the Amide I region (1,600-1700 cm^-1) contain information about secondary structure and require higher concentrations than circular dichroism often with complementary spectral windows. In this pape

SUBMITTER: Pinto Corujo M 

PROVIDER: S-EPMC8830495 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature