Determining vitreous viscosity using fluorescence recovery after photobleaching.

ABSTRACT:

Purpose

Vitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules. The central pig vitreous is thought to closely match human vitreous viscosity. Diffusion is inversely related to viscosity, and diffusion is of fundamental importance for all biochemical reactions. Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules. It would also provide information about vitreous viscosity, which is relevant to drug elimination, and delivery.

Methods

Vitreous viscosity and intravitreal macromolecular diffusio

SUBMITTER: Srikantha N 

PROVIDER: S-EPMC8830689 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature