Acquisition and quantification pipeline for in vivo hyperpolarized ¹³ C MR spectroscopy.

ABSTRACT:

Purpose

The goal of this study was to combine a specialized acquisition method with a new quantification pipeline to accurately and efficiently probe the metabolism of hyperpolarized ¹³ C-labeled compounds in vivo. In this study, we tested our approach on [2-¹³ C]pyruvate and [1-¹³ C]α-ketoglutarate data in rat orthotopic brain tumor models at 3T.

Methods

We used a multiband metabolite-specific radiofrequency (RF) excitation in combination with a variable flip angle scheme to minimize substrate polarization loss and measure fast metabolic processes. We then applied spectral-temporal denoising using singular value decomposition to enhance spectral quality. This was combined with LCModel-based automatic ¹³ C spectral fitting and flip

SUBMITTER: Hong D 

PROVIDER: S-EPMC8830804 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature