Synthesis and use of an amphiphilic dendrimer for siRNA delivery into primary immune cells.

ABSTRACT: Using siRNAs to genetically manipulate immune cells is important to both basic immunological studies and therapeutic applications. However, siRNA delivery is challenging because primary immune cells are often sensitive to the delivery materials and generate immune responses. We have recently developed an amphiphilic dendrimer that is able to deliver siRNA to a variety of cells, including primary immune cells. We provide here a protocol for the synthesis of this dendrimer, as well as siRNA delivery to immune cells such as primary T and B cells, natural killer cells, macrophages, and primary microglia. The dendrimer synthesis entails straightforward click coupling followed by an amidation reaction, and the siRNA delivery protocol requires simple mixing of the siRNA and dendrimer in buffer, w

SUBMITTER: Chen J 

PROVIDER: S-EPMC8830918 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature