Dataset Information

The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2.

ABSTRACT: Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system.

SUBMITTER: Liu J

PROVIDER: S-EPMC8831721 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of <i>Shewanella xiamenensis</i> Lzh-2.

Liu Jian J Liu Kaiquan K Zhao Zhe Z Wang Zheng Z Wang Fengchao F Xin Yuxiu Y Qu Jie J Song Feng F Li Zhenghua Z

Frontiers in microbiology 20220128

Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain <i>Shewanella xiamenensis</i> Lzh-2 is ...[more]

PMID: 35154040

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Project description:BackgroundAs a quorum sensing system, LuxS/AI-2 is closely associated with bacterial growth, biofilm formation, and virulence. As yet, it is not known how the luxS is associated with a diverse array of physiological activities in non- carbapenemase producing carbapenem resistant Escherichia coli (non-CP-CREC). The purpose of this study is to explore the characterization of AI-2/LuxS quorum sensing system in antibiotic resistance, pathogenicity of non-CP-CREC.MethodsA total of five non-CP-CREC isolates that did not have ompC and ompF deletions were collected from various clinical samples from January 2021 to December 2023. RT-qPCR was used to detect genes expression of luxS, acrA, acrB, tolC, mdtB, mdtC, mdtE, mdtF, ompA, ompX, IL-8, IL-6, and TNF-α. Homologous recombination was used to create the luxS knockout strain. Transcriptome sequencing was utilized to analyze gene expression changes before and after the luxS knockout. Biofilm formation was detected using crystal violet staining. Antimicrobial susceptibility test was used to determine drug resistance. Bacterial growth curves were used to detect the influence of the luxS on bacterial growth. A cell infection assay was used to detect the impact of the luxS on bacterial adhesion and the inflammatory response it induces.ResultsOur results indicated that the expression of the luxS was significantly elevated in non-CP-CREC strains compared to the carbapenem antibiotics sensitive E. coli (CSEC), with CREC229 exhibiting the most pronounced difference. Consequently, CREC229 was chosen for the development of the luxS knockout strain (CREC229△luxS). The deletion of the luxS did not impact the growth of non-CP-CREC. RNA sequencing analysis revealed that 82 genes were differentially expressed, with notable alterations observed in genes associated with biofilm formation regulation and outer membrane proteins in the ΔluxS strain. Our transcriptomic results show that the expression of bssS associated with biofilm formation is significantly reduced in the ΔluxS strain, which in turn reduces its capacity for biofilm formation. In addition, the luxS deletion increased the expression of adhesion-related genes, such as ompA and ompX, enhanced HCT-8 adherence to CREC229, and promoted the secretion of the inflammatory cytokine IL-6. In terms of bacterial resistance, the deletion of luxS increased the sensitivity of non-CP-CRECs to aminoglycoside antibiotics.ConclusionsLuxS/AI-2 quorum sensing systems can alter pathogenicity and resistance in several ways.

Dataset Information

The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2.

Publications

The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of <i>Shewanella xiamenensis</i> Lzh-2.

Similar Datasets

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