A Simple and Efficient Method to Cryopreserve Human Ejaculated and Testicular Spermatozoa in -80°C Freezer.

ABSTRACT: Human autologous sperm freezing involves ejaculated sperm, and testicular or epididymal puncture sperm freezing, and autologous sperm freezing is widely used in assisted reproductive technology. In previous studies, researchers have tried to cryopreserve sperm from mammals (rats, dogs, etc.) using a -80°C freezer and have achieved success. It is common to use liquid nitrogen vapor rapid freezing to cryopreserve human autologous sperm. However, the operation of this cooling method is complicated, and the temperature drop is unstable. In this study, we compared the quality of human ejaculation and testicular sperm after liquid nitrogen vapor rapid freezing and -80°C freezing for the first time. By analyzing sperm quality parameters of 93 ejaculated sperm and 10 testicular sperm after liquid

SUBMITTER: Wang X 

PROVIDER: S-EPMC8831890 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature