DRJAMM Is Involved in the Oxidative Resistance in Deinococcus radiodurans.

ABSTRACT: Proteins containing JAB1/MPN/MOV34 metalloenzyme (JAMM/MPN⁺) domains that have Zn²⁺-dependent deubiquitinase (DUB) activity are ubiquitous across among all domains of life. Recently, a homolog in Deinococcus radiodurans, DRJAMM, was reported to possess the ability to cleave DRMoaD-MoaE. However, the detailed biochemical characteristics of DRJAMM in vitro and its biological mechanism in vivo remain unclear. Here, we show that DRJAMM has an efficient in vitro catalytic activity in the presence of Mn²⁺, Ca²⁺, Mg²⁺, and Ni²⁺ in addition to the well-reported Zn²⁺, and strong adaptability at a wide range of temperatures. Disruption of drJAMM led to elevated sensitivity in response to H

SUBMITTER: Cai J 

PROVIDER: S-EPMC8832034 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature