Plant-Derived Cell-Free Biofactories for the Production of Secondary Metabolites.

ABSTRACT: Cell-free expression systems enable the production of proteins and metabolites within a few hours or days. Removing the cellular context while maintaining the protein biosynthesis apparatus provides an open system that allows metabolic pathways to be installed and optimized by expressing different numbers and combinations of enzymes. This facilitates the synthesis of secondary metabolites that are difficult to produce in cell-based systems because they are toxic to the host cell or immediately converted into downstream products. Recently, we developed a cell-free lysate derived from tobacco BY-2 cell suspension cultures for the production of recombinant proteins. This system is remarkably productive, achieving yields of up to 3 mg/mL in a one-pot in vitro transcription-translation r

SUBMITTER: Buntru M 

PROVIDER: S-EPMC8832058 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature