Autophagy Contributes to the Rapamycin-Induced Improvement of Otitis Media.

ABSTRACT: Otitis media (OM) is a pervasive disease that involves hearing loss and severe complications. In our previous study, we successfully established a mouse model of human OM using Tlr2tm1Kir (TLR2^-/-) mice with middle ear (ME) inoculation of streptococcal peptidoglycan-polysaccharide (PGPS). In this study, we found that hearing loss and OM infections in OM mice were significantly alleviated after treatment with rapamycin (RPM), a widely used mechanistic target of RPM complex 1 (mTORC1) inhibitor and autophagy inducer. First of all, we tested the activity of mTORC1 by evaluating p-S6, Raptor, and mTOR protein expression. The data suggested that the protein expression level of p-S6, Raptor and mTOR are decreased in TLR2^-/- mice after the injection of PGPS. Furthermo

SUBMITTER: Xie D 

PROVIDER: S-EPMC8832103 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature