Ryanodine receptor RyR1-mediated elevation of Ca²⁺ concentration is required for the late stage of myogenic differentiation and fusion.

ABSTRACT:

Background

Cytosolic Ca²⁺ plays vital roles in myogenesis and muscle development. As a major Ca²⁺ release channel of endoplasmic reticulum (ER), ryanodine receptor 1 (RyR1) key mutations are main causes of severe congenital myopathies. The role of RyR1 in myogenic differentiation has attracted intense research interest but remains unclear.

Results

In the present study, both RyR1-knockdown myoblasts and CRISPR/Cas9-based RyR1-knockout myoblasts were employed to explore the role of RyR1 in myogenic differentiation, myotube formation as well as the potential mechanism of RyR1-related myopathies. We observed that RyR1 expression was dramatically increased during the late stage of myogenic differentiation, accompanied by significantly elevated cytoplasmic Ca

SUBMITTER: Qiu K 

PROVIDER: S-EPMC8832842 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature