SARS-CoV-2 accessory protein ORF8 is secreted extracellularly as a glycoprotein homodimer.

ABSTRACT: ORF8 is an accessory protein encoded by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consensus regarding the biological functions of ORF8 is lacking, largely because the fundamental characteristics of this protein in cells have not been determined. To clarify these features, we herein established an ORF8 expression system in 293T cells. Using this system, approximately 41% of the ORF8 expressed in 293T cells were secreted extracellularly as a glycoprotein homodimer with inter/intramolecular disulfide bonds. Intracellular ORF8 was sensitive to the glycosidase Endo H, whereas the secreted portion was Endo-H-resistant, suggesting that secretion occurs via a conventional pathway. Additionally, immunoblotting analysis showed that the total amounts of the major histocompatibilit

SUBMITTER: Matsuoka K 

PROVIDER: S-EPMC8832879 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature