Sertoli, Leydig, and Spermatogonial Cells' Specific Gene and Protein Expressions as Dog Testes Evolve from Immature into Mature States.

ABSTRACT: Sertoli, Leydig, and spermatogonial cells proliferate and differentiate from birth to puberty and then stay stable in adulthood. We hypothesized that expressions of spermatogenesis-associated genes are not enhanced with a mere increase of these cells' numbers. To accept this postulation, we investigated the abundances of Sertoli cell-specific FSHR and AMH, Leydig cell-specific LHR and INSL3, and spermatogonia-specific THY1 and CDH1 markers in immature and mature canine testis. Four biological replicates of immature and mature testes were processed, and RT-PCR was performed to elucidate the cells' specific markers. The data were analyzed by ANOVA, using the 2^-∆∆Ct method to ascertain differences in mRNA expressions. In addition, Western b

SUBMITTER: Kasimanickam VR 

PROVIDER: S-EPMC8833615 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature