Antibody Generation and Rapid Immunochromatography Using Time-Resolved Fluorescence Microspheres for Propiconazole: Fungicide Abused as Growth Regulator in Vegetable.

ABSTRACT: Propiconazole (PCZ) is a fungicide popularly used to prevent and control wheat and rice bakanae disease, etc. However, it was recently found to be illegally employed as a plant regulator to induce thick stems and dark green leaves of Brassica campestris, a famous vegetable in Guangdong, South China. Due to a lack of available recognition molecules to the target analyte, it is still a big challenge to establish a rapid surveillance screening method. In this study, a novel chiral hapten was rationally designed, and an artificial immunogen was then prepared for the generation of a specific antibody against propiconazole for the first time. Using the obtained antibody, a highly sensitive time-resolved fluorescence microspheres lateral flow immunochromatographic assay (TRFMs-LFIA) was establish

SUBMITTER: Chen B 

PROVIDER: S-EPMC8834100 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature