Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures.

ABSTRACT: The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models

SUBMITTER: Kreß S 

PROVIDER: S-EPMC8834321 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature