Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers.

ABSTRACT: Myeloid progenitors are intermediates between Hematopoietic Stem Cells (HSCs) and Myeloid effector progeny. In mouse bone marrow, they are part of the Lineage^- cKit⁺ Sca1^- (LK) compartment. To date, most researchers used CD34 and FcγR surface markers for the dissection of this compartment into various populations. Surprisingly, however, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we suggest using CD150 instead of FcγR. We re-analyzed published single-cell RNA-Seq data and found that CD34/CD150 provides better sub-populations separation, compared to the "classical" CD34/FcγR-based approach. We confirm our findings by independent FACS analysis. We demonstrate comparable differentiation poten

SUBMITTER: Olender L 

PROVIDER: S-EPMC8834359 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature