Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen.

ABSTRACT: Fibrin forms the structural scaffold of blood clots and has great potential for biomaterial applications. Creating recombinant expression systems of fibrinogen, fibrin's soluble precursor, would advance the ability to construct mutational libraries that would enable structure-function studies of fibrinogen and expand the utility of fibrin as a biomaterial. Despite these needs, recombinant fibrinogen expression systems, thus far, have relied on the time-consuming creation of stable cell lines. Here we present tests of a transient fibrinogen expression system that can rapidly generate yields of 8-12 mg/L using suspension HEK Expi293^TM cells. We report results from two different plasmid systems encoding the fibrinogen cDNAs and two different transfection reagents. In addition, we d

SUBMITTER: Popovic G 

PROVIDER: S-EPMC8835685 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature