Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro.

ABSTRACT: Extracellular vesicles (EVs) play an important role in intercellular communication and are involved in both physiological and pathological processes. In the central nervous system (CNS), EVs secreted from different brain cell types exert a sundry of functions, from modulation of astrocytic proliferation and microglial activation to neuronal protection and regeneration. However, the effect of aging on the biological functions of neural EVs is poorly understood. In this work, we studied the biological effects of small EVs (sEVs) isolated from neural cells maintained for 14 or 21 days in vitro (DIV). We found that EVs isolated from 14 DIV cultures reduced the extracellular levels of lactate dehydrogenase (LDH), the expression levels of the astrocytic protein GFAP, and the complexity of astroc

SUBMITTER: Almansa D 

PROVIDER: S-EPMC8835866 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature