Determination of the Optimal Bacterial DNA Extraction Method to Explore the Urinary Microbiota.

ABSTRACT: Recent advances in molecular biology have been successfully applied to the exploration of microbiota from various fluids. However, the urinary microbiota remains poorly explored, as its analysis requires specific technical considerations. Indeed, urine is a low microbial biomass environment, in which the representativity of each bacterium must be respected to obtain accurate data. Thus, sensitive extraction methods must be used to obtain good quality DNA while preserving the proportions between species. To address this, we compared the efficiency of five extraction methods on artificial urine samples spiked with low amounts of four bacteria species. The quality of the DNA obtained was further evaluated by different molecular biology approaches, including quantitative PCR and amplicon-based

SUBMITTER: Vendrell JA 

PROVIDER: S-EPMC8835916 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature