RT-qPCR Detection of SARS-CoV-2: No Need for a Dedicated Reverse Transcription Step.

ABSTRACT: Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real-time, fluorescence-based usage (RT-qPCR), it has long been a core technology driving the accurate, rapid and sensitive laboratory diagnosis of infectious diseases. However, RT-qPCR protocols have changed little over the past 30 years, with the RT step constituting a significant percentage of the time taken to complete a typical RT-qPCR assay. When applied to research investigations, reverse transcription has been evaluated by criteria such as maximum yield, length of transcription, fidelity, and faithful representation of an RNA pool. Cruciall

SUBMITTER: Bustin SA 

PROVIDER: S-EPMC8835954 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature