Structural Basis of Redox-Sensing Transcriptional Repressor Rex with Cofactor NAD⁺ and Operator DNA.

ABSTRACT: The transcriptional repressor Rex plays important roles in regulating the expression of respiratory genes by sensing the reduction-oxidation (redox) state according to the intracellular NAD⁺/NADH balance. Previously, we reported on crystal structures of apo, NAD⁺-bound, and NADH-bound forms of Rex from Thermotoga maritima to analyze the structural basis of transcriptional regulation depending on either NAD⁺ or NADH binding. In this study, the crystal structure of Rex in ternary complex with NAD⁺ and operator DNA revealed that the N-terminal domain of Rex, including the helix-turn-helix motif, forms extensive contacts with DNA in addition to DNA sequence specificity. Structural comparison of the Rex in apo, NAD⁺-bound, NAD

SUBMITTER: Jeong KH 

PROVIDER: S-EPMC8836258 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature