The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrates.

ABSTRACT: Catch bonds are a form of mechanoregulation wherein protein-ligand interactions are strengthened by the application of dissociative tension. Currently, the best-characterized examples of catch bonds are between single protein-ligand pairs. The essential AAA (ATPase associated with diverse cellular activities) mechanoenzyme Mdn1 drives at least two separate steps in ribosome biogenesis, using its MIDAS domain to extract the ubiquitin-like (UBL) domain-containing proteins Rsa4 and Ytm1 from ribosomal precursors. However, it must subsequently release these assembly factors to reinitiate the enzymatic cycle. The mechanism underlying the switching of the MIDAS-UBL interaction between strongly and weakly bound states is unknown. Here, we use optical tweezers to investigate the force dependence o

SUBMITTER: Mickolajczyk KJ 

PROVIDER: S-EPMC8837202 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature