Identification of circRNA Expression Profiles in BMSCs from Glucocorticoid-Induced Osteoporosis Model.

ABSTRACT:

Background

Circular RNAs (circRNAs) contribute to the regulation of many diseases. However, little is known about the role of circRNAs in the development of glucocorticoid-induced osteoporosis (GIOP). The present study is aimed at systematically characterizing the circRNA expression profiles in GIOP and predict the potential functions of the associated regulatory networks.

Methods

A small animal GIOP model was developed in Sprague-Dawley rats given daily intraperitoneal doses of the synthetic glucocorticoid dexamethasone. Micro-CT and bone histomorphometry were performed to characterize the bone loss. Alizarin red S (ARS) staining and alkaline phosphatase (ALP) activity were assessed to determine the osteogenic differentiation potential of BMSCs. RNA sequencing was performed

SUBMITTER: Chen Z 

PROVIDER: S-EPMC8837445 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature