Effects of butyrate^- on ruminal Ca²⁺ transport: evidence for the involvement of apically expressed TRPV3 and TRPV4 channels.

ABSTRACT: The ruminal epithelium absorbs large quantities of NH₄⁺ and Ca²⁺. A role for TRPV3 has emerged, but data on TRPV4 are lacking. Furthermore, short-chain fatty acids (SCFA) stimulate ruminal Ca²⁺ and NH₄⁺ uptake in vivo and in vitro, but the pathway is unclear. Sequencing of the bovine homologue (bTRPV4) revealed 96.79% homology to human TRPV4. Two commercial antibodies were tested using HEK-293 cells overexpressing bTRPV4, which in ruminal protein detected a weak band at the expected ~ 100 kDa and several bands ≤ 60 kDa. Immunofluorescence imaging revealed staining of the apical membrane of the stratum granulosum for bTRPV3 and bTRPV4, with cytosolic staining in other layers of the ruminal epithelium. A similar expression pat

SUBMITTER: Liebe F 

PROVIDER: S-EPMC8837523 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature