Development of a proof-of-concept microfluidic portable pathogen analysis system for water quality monitoring.

ABSTRACT: Waterborne diseases cause millions of deaths worldwide, especially in developing communities. The monitoring and rapid detection of microbial pathogens in water is critical for public health protection. This study reports the development of a proof-of-concept portable pathogen analysis system (PPAS) that can detect bacteria in water with the potential application in a point-of-sample collection setting. A centrifugal microfluidic platform is adopted to integrate bacterial cell lysis in water samples, nucleic acid extraction, and reagent mixing with a droplet digital loop mediated isothermal amplification assay for bacteria quantification onto a single centrifugal disc (CD). Coupled with a portable "CD Driver" capable of automating the assay steps, the CD functions as a single step bacteria

SUBMITTER: Gowda HN 

PROVIDER: S-EPMC8837627 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature