Validation of the 24,25-dihydroxyvitamin D₃ to 25-hydroxyvitamin D₃ ratio as a biomarker of 25-hydroxyvitamin D₃ clearance.

ABSTRACT: The formation of 24,25-dihydroxyvitamin D (24,25(OH)₂D) from 25-hydroxyvitamin D (25(OH)D) is the primary mechanism for the metabolic clearance of 25(OH)D, and is regulated by tissue-level vitamin D activity. The ratio of 24,25(OH)₂D₃ to 25(OH)D₃ in blood (vitamin D metabolite ratio, VDMR) is postulated to be a marker of 25(OH)D₃ clearance, however this has never been tested. We measured baseline 24,25(OH)₂D₃ and 25(OH)D₃ concentrations in 87 participants by liquid chromatography-tandem mass spectrometry. Following an infusion of deuterated 25(OH)D₃, blood samples for each participant were collected over 56 days and analyzed for deuterated vitamin D metabolites. 25(OH)D₃ clearance

SUBMITTER: Hsu S 

PROVIDER: S-EPMC8837693 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature