Bioremediation of motor oil-contaminated soil and water by a novel indigenous Pseudomonas otitidis strain DU13 and characterization of its biosurfactant.

ABSTRACT: Production of biosurfactant by a novel indigenous isolate Pseudomonas otitidis strain DU13 and its role in bioremediation of petroleum hydrocarbon is reported. The identity of the isolate was confirmed by 16S rDNA gene sequencing analysis (Genbank accession: MK177190). The biosurfactant produced by the isolate could reduce the surface tension of petroleum supplemented medium by 46% just after 7 days of treatment. The emulsification index (E ₂₄ ) of the surfactant was found 37, 35, and 20%, respectively, against used motor oil, diesel, and kerosene. The FTIR spectrum of the crude biosurfactant showed the presence of υ_C-H stretch, υ_CH2, υ_-C=C stretch and υ_C-H bonding. The isolated strain could degrade 26% of TPH cont

SUBMITTER: Gogoi B 

PROVIDER: S-EPMC8837742 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature