Xenogeneic-Free Human Intestinal Organoids for Assessing Intestinal Nutrient Absorption.

ABSTRACT: Since many nutrients, including the three major ones of glucose, dipeptides, and cholesterol, are mainly absorbed in the small intestine, the assessment of their effects on intestinal tissue is important for the study of food absorption. However, cultured intestinal cell lines, such as Caco-2 cells, or animal models, which differ from normal human physiological conditions, are generally used for the evaluation of intestinal absorption and digestion. Therefore, it is necessary to develop an alternative in vitro method for more accurate analyses. In this study, we demonstrate inhibitory effects on nutrient absorption through nutrient transporters using three-dimensional xenogeneic-free human intestinal organoids (XF-HIOs), with characteristics of the human intestine, as we previously reporte

SUBMITTER: Inoue M 

PROVIDER: S-EPMC8838315 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature