Evaluation of Fast and Sensitive Proteome Profiling of FF and FFPE Kidney Patient Tissues.

ABSTRACT: The application of proteomics to fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) human tissues is an important development spurred on by requests from stakeholder groups in clinical fields. One objective is to complement current diagnostic methods with new specific molecular information. An important goal is to achieve adequate and consistent protein recovery across and within large-scale studies. Here, we describe development of several protocols incorporating mass spectrometry compatible detergents, including Rapigest, PPS, and ProteaseMax. Methods were applied on 4 and 15 μm thick FF tissues, and 4 μm thick FFPE tissues. We evaluated sensitivity and repeatability of the methods and found that the protocol containing Rapigest enabled detection of 630 proteins from FF tissue

SUBMITTER: Dapic I 

PROVIDER: S-EPMC8838561 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature