Dataset Information

Morphology Characterization, Molecular Identification, and Pathogenicity of Fungal Pathogen Causing Kaffir Lime Leaf Blight in Northern Thailand.

ABSTRACT: Thailand is known to be the largest producer of kaffir lime leaf products in the global market. In 2021, leaf blight was found on kaffir lime plants (Citrus hystrix DC.) in Lamphun Province of northern Thailand. This disease has been associated with significant economic losses. However, there have been no prior reports of leaf blight on kaffir lime plants in Thailand or anywhere else in the world. In this study, causal fungi were isolated from lesions of kaffir lime plants and a total of three fungal isolates were obtained. All causal fungi were identified as Lasiodiplodia chinensis based on morphological characteristics and the phylogenetic analysis of combined sequences of the internal transcribed spacer (ITS) of ribosomal DNA, the translation elongation factor 1-alpha (tef-1), β-tubulin (tub), and RNA polymerase II subunit (rbp2) genes. Pathogenicity tests were conducted and the results revealed that all isolated fungi caused symptoms of leaf blight on inoculated leaves. This outcome was similar to symptoms that naturally occur and have been observed in the field. This is the first report on kaffir lime leaf blight caused by L. chinensis. Our study will provide information of high value for the development of effective strategies for the monitoring and prevention of this disease.

SUBMITTER: Suwannarach N

PROVIDER: S-EPMC8838810 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Morphology Characterization, Molecular Identification, and Pathogenicity of Fungal Pathogen Causing Kaffir Lime Leaf Blight in Northern Thailand.

Suwannarach Nakarin N Khuna Surapong S Kumla Jaturong J Cheewangkoon Ratchadawan R Suttiprapan Piyawan P Lumyong Saisamorn S

Plants (Basel, Switzerland) 20220120 3

Thailand is known to be the largest producer of kaffir lime leaf products in the global market. In 2021, leaf blight was found on kaffir lime plants (<i>Citrus hystrix</i> DC.) in Lamphun Province of northern Thailand. This disease has been associated with significant economic losses. However, there have been no prior reports of leaf blight on kaffir lime plants in Thailand or anywhere else in the world. In this study, causal fungi were isolated from lesions of kaffir lime plants and a total of ...[more]

PMID: 35161253

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Project description:Foliar diseases of maize cause severe economic losses in India and around the world. The increasing severity of maize leaf blight (MLB) over the past ten years necessitates rigorous identification and characterization of MLB-causing pathogens from different maize production zones to ensure the success of resistance breeding programs and the selection of appropriate disease management strategies. Although Bipolaris maydis is the primary pathogen causing MLB in India, other related genera such as Curvularia, Drechslera, and Exserohilum, and a taxonomically distant genus, Alternaria, are known to infect maize in other countries. To investigate the diversity of pathogens associated with MLB in India, 350 symptomatic leaf samples were collected between 2016 and 2018, from 20 MLB hotspots in nine states representing six ecological zones where maize is grown in India. Twenty representative fungal isolates causing MLB symptoms were characterized based on cultural, pathogenic, and molecular variability. Internal Transcribed Spacer (ITS) and glyceraldehyde-3-phosphate dehydrogenase (GADPH) gene sequence-based phylogenies showed that the majority of isolates (13/20) were Bipolaris maydis. There were also two Curvularia papendorfii isolates, and one isolate each of Bipolaris zeicola, Curvularia siddiquii, Curvularia sporobolicola, an unknown Curvularia sp. isolate phylogenetically close to C. graminicola, and an Alternaria sp. isolate. The B. zeicola, the aforesaid four Curvularia species, and the Alternaria sp. are the first reports of these fungi causing MLB in India. Pathogenicity tests on maize plants showed that isolates identified as Curvularia spp. and Alternaria sp. generally caused more severe MLB symptoms than those identified as Bipolaris spp. The diversity of fungi causing MLB, types of lesions, and variation in disease severity by different isolates described in this study provide baseline information for further investigations on MLB disease distribution, diagnosis, and management in India.

Dataset Information

Morphology Characterization, Molecular Identification, and Pathogenicity of Fungal Pathogen Causing Kaffir Lime Leaf Blight in Northern Thailand.

Publications

Morphology Characterization, Molecular Identification, and Pathogenicity of Fungal Pathogen Causing Kaffir Lime Leaf Blight in Northern Thailand.

Similar Datasets

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