Sunset Yellow Dye Induces Amorphous Aggregation in β-Lactoglobulin at Acidic pH: A Multi-Techniques Approach.

ABSTRACT: Protein aggregation is of two types: (i) amorphous and (ii) amyloid fibril. Several extrinsic factors (temperature, pH, and small ligands) stimulate protein aggregation in vitro. In this study, we have examined the role of sunset yellow (SY) on the β-lactoglobulin (BLG) aggregation at pH 2.0. We have used spectroscopic (turbidity, Rayleigh light scattering (RLS), far-UV CD) and microscopic (transmission electron microscopy [TEM]) techniques to describe the effects of SY on BLG aggregation. Our results showed that BLG aggregation is dependent on SY concentrations. Very low concentrations (0.0-0.07 mM) of SY were unable to induce aggregation, while SY in the concentrations range of 0.1-5.0 mM induces aggregation in BLG. The kinetics of SY-stimulated aggregation is very fast and monomeric for

SUBMITTER: Khan JM 

PROVIDER: S-EPMC8839080 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature