Dataset Information

Cholesteric Chiral Molecular Tweezer for Rapid Detection of F- in Food Samples.

ABSTRACT: Chiral cholesteric molecular tweezer 7d was synthesized and its influences on changes in the ultraviolet (UV) and fluorescence spectra of various anions were investigated. The results displayed that molecular tweezer 7d selectively recognized F- ions in dimethyl sulfoxide with a detection limit of 5.14 μmol/L, while other anions had little interference. On this basis, a method for the rapid detection of F- ions by host molecular tweezer 7d was established, and the naked-eye detection of F- was realized through the unique yellow color of the complex solution. According to the determination of F- ions in real food samples, it was proved that the established method had good application prospects in F- ion detection.

SUBMITTER: Liu Z

PROVIDER: S-EPMC8839199 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Cholesteric Chiral Molecular Tweezer for Rapid Detection of F- in Food Samples.

Liu Zhe Z Wang Hong H Chen Zhu Z Ye Ying Y Wang Yuwei Y

Molecules (Basel, Switzerland) 20220207 3

Chiral cholesteric molecular tweezer 7d was synthesized and its influences on changes in the ultraviolet (UV) and fluorescence spectra of various anions were investigated. The results displayed that molecular tweezer 7d selectively recognized F- ions in dimethyl sulfoxide with a detection limit of 5.14 μmol/L, while other anions had little interference. On this basis, a method for the rapid detection of F- ions by host molecular tweezer 7d was established, and the naked-eye ...[more]

PMID: 35164363

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Project description:The utility of pesticides in the agricultural field is unquestionable, but at the same time pesticide use presents serious hazards to the environment and the human health. For that reason, detection of pesticides and their biotransformation products in food is of utmost importance. According to previous studies, esterase-based biosensors have been proposed as a viable and efficient solution for the detection of organophosphate pesticides. In this project, a double mutant of the thermostable esterase-2 (EST2) from Alicyclobacillus acidocaldarius was studied as a potential biosensor, for its ability to detect residual amounts of pesticides. Initial characterisation of the enzyme was performed, that included determination of optimal pH, thermophilicity, as well as kinetic analysis. Subsequently, the enzyme was studied by enzymatic activity assays with and without the presence of various organophosphate compounds. The effect of the organophosphates on the enzymatic activity was measured and complete inhibition of the enzyme was observed after incubation with paraoxon. These experiments were followed by an additional method involving labelling of the enzyme with a fluorescent probe. In this case, the effect of different pesticides on the EST2 enzyme was monitored by measuring the fluorescence quenching upon addition to the enzyme. Fourteen compounds were screened with this method and significant fluorescence quenching was observed in the presence of paraoxon and methyl-paraoxon when used in equimolar amounts with the enzyme in the range of nanomolar. This biosensor has been also used to test the presence of pesticides in real food samples, like fruits and juices. This research represents a starting point to develop effective fluorescence-based biosensors aiming at the screening of mutants with different pesticide selectivity profiles. The use of this enzyme-based biosensor can have applications in the field of food traceability as well as environmental monitoring, to control the presence of toxic chemicals, in particular organophosphate pesticides.

Dataset Information

Cholesteric Chiral Molecular Tweezer for Rapid Detection of F- in Food Samples.

Publications

Cholesteric Chiral Molecular Tweezer for Rapid Detection of F<sup>-</sup> in Food Samples.

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