Semi-Quantitative MALDI Measurements of Blood-Based Samples for Molecular Diagnostics.

ABSTRACT: Accurate and precise measurement of the relative protein content of blood-based samples using mass spectrometry is challenging due to the large number of circulating proteins and the dynamic range of their abundances. Traditional spectral processing methods often struggle with accurately detecting overlapping peaks that are observed in these samples. In this work, we develop a novel spectral processing algorithm that effectively detects over 1650 peaks with over 3.5 orders of magnitude in intensity in the 3 to 30 kD m/z range. The algorithm utilizes a convolution of the peak shape to enhance peak detection, and accurate peak fitting to provide highly reproducible relative abundance estimates for both isolated peaks and overlapping peaks. We demonstrate a substantial increase in the reprodu

SUBMITTER: Koc MA 

PROVIDER: S-EPMC8840133 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature