Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development.

ABSTRACT:

Background

The Natterin protein family was first discovered in the venom of the medically significant fish Thalassophryne nattereri, and over the last decade natterin-like genes have been identified in various organisms, notably performing immune-related functions. Previous findings support natterin-like genes as effector defense molecules able to activate multiprotein complexes driving the host innate immune response, notably due to the pore-forming function of the aerolysin superfamily members. Herein, employing a combination of the CRISPR/Cas9 depletion system, phenotype-based screening, and morphometric methods, we evaluated the role of one family member, LOC795232, in the embryonic development of zebrafish since it might be implicated in multiple roles and characterization of

SUBMITTER: Seni-Silva AC 

PROVIDER: S-EPMC8840632 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature