Secretory protein beta-lactoglobulin in cattle stable dust may contribute to the allergy-protective farm effect.

ABSTRACT:

Background

Growing up on a cattle farm and consuming raw cow's milk protects against asthma and allergies. We expect a cattle-specific protein as active component in this farm effect.

Methods

Dust was collected from cattle and poultry stables and from mattresses of households. Urine was obtained from cattle, and ambient aerosols were sampled. Samples were analysed for BLG by SDS PAGE/immunoblot and mass spectrometry, and for association with metals by SEC-ICP-MS. PBMC of healthy donors were incubated with BLG +/- zinc, and proliferation and cytokines determined. BALB/c mice were pre-treated intranasally with stable dust extract containing BLG or depleted of BLG, and subsequent allergy response after sensitization was evaluated on antibody and symptom level.

Results

A

SUBMITTER: Pali-Scholl I 

PROVIDER: S-EPMC8840802 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature