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Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.

ABSTRACT: Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.

SUBMITTER: Yugueros J 

PROVIDER: S-EPMC88409 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature

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Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.

Yugueros J J   Temprano A A   Sánchez M M   Luengo J M JM   Naharro G G  

Journal of clinical microbiology 20011001 10

Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity. ...[more]

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