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Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation.

ABSTRACT: SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop a preclinical model for recapitulating SARS-CoV-2 pathogenesis is still urgent and essential for the control of the pandemic. Here, we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration. We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar units, which lays a fundamental basis for viral infection studies at the organ level. Using virus-analogous chemicals and pseudovirus, we have explored virus pathogenesis and blocking ability of antibodies during viral infection. This work provides a favorable platform for SARS-CoV-2-related researches and has a great potential for physiology and pathophysiology studies of the human lung at the organ level in vitro.

SUBMITTER: Cao T

PROVIDER: S-EPMC8841031 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation.

Cao Ting T Shao Changmin C Yu Xiaoyu X Xie Ruipei R Yang Chen C Sun Yulong Y Yang Shaohua S He Wangjian W Xu Ye Y Fan Qihui Q Ye Fangfu F

Research (Washington, D.C.) 20220204

SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop a preclinical model for recapitulating SARS-CoV-2 pathogenesis is still urgent and essential for the control of the pandemic. Here, we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration. We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar un ...[more]

PMID: 35224503

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