E-Selectin-Overexpressing Mesenchymal Stem Cell Therapy Confers Improved Reperfusion, Repair, and Regeneration in a Murine Critical Limb Ischemia Model.

ABSTRACT:

Aims

Novel cell-based therapeutic angiogenic treatments for patients with critical limb ischemia may afford limb salvage. Mesenchymal stem cells (MSCs) do not overexpress E-selectin; however, we have previously demonstrated the cell-adhesion molecule's vital role in angiogenesis and wound healing. Thus, we created a viral vector to overexpress E-selectin on MSCs to increase their therapeutic profile.

Methods and results

Femoral artery ligation induced hind limb ischemia in mice and intramuscular injections were administered of vehicle or syngeneic donor MSCs, transduced ex vivo with an adeno-associated viral vector to express either GFP⁺ (MSC^GFP) or E-selectin-GFP⁺ (MSC^{E-selectin-GFP}). Laser Doppler Imaging demonstrated sign

SUBMITTER: Quiroz HJ 

PROVIDER: S-EPMC8841646 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature