Circ_0026134 promotes NSCLC progression by the miR-3619-5p/CHAF1B axis.

ABSTRACT:

Background

Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Circular RNAs (circRNAs) have been implicated in the pathogenesis of NSCLC. In this study, we explored the molecular determinants underlying the oncogenic property of circ_0026134 in NSCLC.

Methods

The levels of circ_0026134, miR-3619-5p and chromatin assembly factor 1 subunit B axis (CHAF1B) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Cell colony formation, migration, invasion and apoptosis were detected by colony formation, Transwell, and flow cytometry assays, respectively. Direct relationships among circ_0026134, miR-3619-5p and CHAF1B were verified by dual-luciferase reporter assays.

Results

Our results showed that circ_0

SUBMITTER: Ge L 

PROVIDER: S-EPMC8841691 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature