The Resistance Mechanism Governs Physiological Adaptation of Escherichia coli to Growth With Sublethal Concentrations of Carbapenem.

ABSTRACT: Factors governing resistance in carbapenem-resistant Enterobacteriaceae are manifold. Despite ample research efforts, underlying molecular mechanisms are still only partly understood. Furthermore, little is known on (eco)physiological consequences from resistance acquisition originating from distinct mechanisms in respective bacteria. In this study, we examined physiological adaptation of Escherichia coli clinical isolates exhibiting two distinct resistance mechanisms-either carrying a carbapenemase (n = 4, CARB) or alterations in porin-encoding genes (n = 6, POR)-during growth with sublethal concentrations of ertapenem in chemostat culture. Basic growth parameters based on optical density and flow-cytometric analyses as well as global gene expression patterns u

SUBMITTER: Schafer F 

PROVIDER: S-EPMC8841762 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature