Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation.

ABSTRACT: Due to the various biological activities of chitosan oligosaccharides (COSs), they have great potential value for use in many areas. Chitosanase plays an important role in enzymatic preparation of COSs. Herein, a gene encoding a chitosanase (ShCsn46) from marine Streptomyces hygroscopicus R1 was cloned and the sequences encoding ShCsn46 without signal peptide were optimized based on the codon usage of Pichia pastoris (P. pastoris). In addition, the optimized gene was ligated to pPICZαA and transformed to P. pastoris X33. After screening, a recombinant strain named X33-Sh33 with the highest activity was isolated from 96 recombinant colonies. The maximum activity and total protein concentration of the recombinant strain ShCsn46 were 2250 U/ml

SUBMITTER: Wang J 

PROVIDER: S-EPMC8841797 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature