Large-scale analysis of Drosophila core promoter function using synthetic promoters.

ABSTRACT: The core promoter plays a central role in setting metazoan gene expression levels, but how exactly it "computes" expression remains poorly understood. To dissect its function, we carried out a comprehensive structure-function analysis in Drosophila. First, we performed a genome-wide bioinformatic analysis, providing an improved picture of the sequence motifs architecture. We then measured synthetic promoters' activities of ~3,000 mutational variants with and without an external stimulus (hormonal activation), at large scale and with high accuracy using robotics and a dual luciferase reporter assay. We observed a strong impact on activity of the different types of mutations, including knockout of individual sequence motifs and motif combinations, variations of motif strength, nucleosome pos

SUBMITTER: Qi Z 

PROVIDER: S-EPMC8842121 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature