N-glycan profiling alterations of serum and immunoglobulin G in immune thrombocytopenia.

ABSTRACT:

Background

The glycosylation alterations of serum and IgG are involved in a variety of autoimmune and inflammatory diseases and have shown great potential in biomarker field. The diagnosis of immune thrombocytopenia (ITP) is exclusive. Our study aimed to discover the potential glyco-biomarkers for auxiliary diagnosis of ITP.

Methods

The serum samples were obtained from 61 ITP patients and 35 healthy controls, and IgG samples were purified from 34 out of 61 ITP patients and 35 healthy controls. DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) was used to analyze serum and IgG N-glycan profiling.

Results

6 of 12 serum N-glycan peaks, 6 of 7 IgG N-glycan peaks, serum fucosylation, and IgG galactosylation were significantly different bet

SUBMITTER: Wang W 

PROVIDER: S-EPMC8842136 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature