The Two Faces of the Liquid Ordered Phase.

ABSTRACT: Coexisting liquid ordered (L_o) and liquid disordered (L_d) lipid phases in synthetic and plasma membrane-derived vesicles are commonly used to model the heterogeneity of biological membranes, including their putative ordered rafts. However, raft-associated proteins exclusively partition to the L_d and not the L_o phase in these model systems. We believe that the difference stems from the different microscopic structures of the lipid rafts at physiological temperature and the L_o phase studied at room temperature. To probe this structural diversity across temperatures, we performed atomistic molecular dynamics simulations, differential scanning calorimetry, and fluorescence spectroscopy on L_o phase membranes. Our results suggest

SUBMITTER: Schachter I 

PROVIDER: S-EPMC8842317 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature