Chronic E-Cigarette Exposure Alters Human Alveolar Macrophage Morphology and Gene Expression.

ABSTRACT:

Introduction

Alveolar macrophages (AMs) are lung-resident immune cells that phagocytose inhaled particles and pathogens, and help coordinate the lung's immune response to infection. Little is known about the impact of chronic e-cigarette use (ie, vaping) on this important pulmonary cell type. Thus, we determined the effect of vaping on AM phenotype and gene expression.

Aims and methods

We recruited never-smokers, smokers, and e-cigarette users (vapers) and performed research bronchoscopies to isolate AMs from bronchoalveolar lavage fluid samples and epithelial cells from bronchial brushings. We then performed morphological analyses and used the Nanostring platform to look for changes in gene expression.

Results

AMs obtained from smokers and vapers were phenotypically

SUBMITTER: Davis ES 

PROVIDER: S-EPMC8842413 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature