Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragments.

ABSTRACT: While glycans are present on the surface of cells in all living organisms and play key roles in most biological processes, their isomeric complexity makes their structural characterization challenging. Of particular importance are positional isomers, for which analytical standards are difficult to obtain. We combine ultrahigh-resolution ion-mobility spectrometry with collision-induced dissociation and cryogenic infrared spectroscopy to determine the structure of N-glycan positional isomers. This approach is based on first separating the parent molecules by SLIM-based IMS, producing diagnostic fragments specific to each positional isomer, separating the fragments by IMS, and identifying them by comparing their IR fingerprints to a previously recorded spectral database. We demonstrate

SUBMITTER: Bansal P 

PROVIDER: S-EPMC8842669 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature