Product Studies and Mechanistic Analysis of the Reaction of Methylglyoxal with Deoxyguanosine.

ABSTRACT: Methylglyoxal (MG) is a highly reactive electrophile produced endogenously as a byproduct of glucose metabolism and protein catabolism and exogenously as a food contaminant. MG reacts spontaneously with proteins, lipids, and nucleic acids to form advanced glycation end products (AGEs), modifying or inhibiting their function. Protein AGEs are associated with pathological complications of diabetes, cancer, and neurodegenerative diseases, while the physiological impact of DNA, RNA, and lipid AGE formation is less well explored. Conflicting reports in the literature on the biologically significant DNA-AGE product distribution and mechanisms of formation prompted a re-examination of the reaction products of MG with dG, oligonucleotides, and plasmid DNA under varying conditions of MG:dG stoichio

SUBMITTER: Shuck SC 

PROVIDER: S-EPMC8842848 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature