METTL14-mediated m⁶A modification of circORC5 suppresses gastric cancer progression by regulating miR-30c-2-3p/AKT1S1 axis.

ABSTRACT:

Background

N6-methyladenosine (m⁶A) RNA methylation and circular RNAs (circRNAs) have been shown to act vital roles in multiple malignancies including gastric cancer (GC). However, there is little knowledge about how m⁶A modification of circRNAs contributes to GC progression.

Methods

The association of METTL14 expression with the clinicopathological characteristics and prognosis in patients with GC was assessed by Western blot, Immunohistochemistry and public datasets. In vitro and vivo function experiments were conducted to investigate the role of METTL14 in GC. Furthermore, m⁶A-circRNA epitranscriptomic microarray was utilized to identify METTL14-mediated m⁶A modification of circRNAs, which were validated by methylated RNA immuno

SUBMITTER: Fan HN 

PROVIDER: S-EPMC8842906 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature