Role of ammonia for brain abnormal protein glycosylation during the development of hepatitis B virus-related liver diseases.

ABSTRACT:

Background

Ammonia is the most typical neurotoxin in hepatic encephalopathy (HE), but the underlying pathophysiology between ammonia and aberrant glycosylation in HE remains unknown.

Results

Here, we used HBV transgenic mice and astrocytes to present a systems-based study of glycosylation changes and corresponding enzymes associated with the key factors of ammonia in HE. We surveyed protein glycosylation changes associated with the brain of HBV transgenic mice by lectin microarrays. Upregulation of Galβ1-3GalNAc mediated by core 1 β1,3-galactosyltransferase (C1GALT1) was identified as a result of ammonia stimulation. Using in vitro assays, we validated that upregulation of C1GALT1 is a driver of deregulates calcium (Ca²⁺) homeostasis by overexpression of inositol

SUBMITTER: Yang J 

PROVIDER: S-EPMC8842931 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature